破伤风毒素片段C分子不具备神经毒毒性，在动物实验中可诱导小鼠产生中和抗体，并能保护小鼠抗破伤风毒素攻击，是理想的亚单位疫苗候选者。我们用基因工程技术在大肠杆菌中重组表达破伤风毒素片段C抗原，为研制新型破伤风疫苗打下基础。从破伤风梭状芽胞杆菌中提取其总DNA，取 Tetanus toxin fragment C molecules are not neurotoxic, in mice can induce neutralizing antibodies and protect mice against tetanus toxin attack, is an ideal subunit vaccine candidate. We recombinantly expressed tetanus toxin fragment C antigen in Escherichia coli by genetic engineering, which laid the foundation for the development of a new tetanus vaccine. Clostridium tetani extract its total DNA, take