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人骨髓细胞体外长期培养基质层细胞按其核浆、形态特征分为四类：ＡＣ１最大，有数个明显核仁，胞浆呈薄纱状；ＡＣ２与巨噬细胞类似，核小深染，胞内有吞噬物；ＡＣ３最显著的特征是胞浆内广泛分布的脂质空泡；ＡＣ４最小，核大深染，胞浆少。从培养第４周至第８周，ＡＣ１及ＡＣ２分别从２１．７５％、３１．２５％上升至４０．５％、５５％；ＡＣ３及ＡＣ４分别从３１％、１５．５％降至１．５％、３％。碱性磷酸酶－抗碱性磷酸酶（ＡＰＡＡＰ）法检测细胞表面抗原，可见ＡＣ１纤维联结蛋白阳性，ＡＣ２的ＣＤ６８强阳性，ＡＣ３的纤维联结蛋白弱阳性，ＡＣ４的上两种单抗及因子ＶＩＩＩ相关活性抗原均标记阴性。本实验结果可作为一个参考值，衡量在各种实验条件下基质层细胞组成的变化。
Human bone marrow cells in vitro long-term culture of stromal cells according to their nuclear plasma, morphological characteristics are divided into four categories: AC1 maximum, a few obvious nucleoli, cytoplasm was thin gauze; AC2 and macrophages, There are phagocytosis; AC3 most prominent feature is widely distributed in the cytoplasm of lipid vacuoles; AC4 smallest, deep nuclei dyed, less cytoplasm. The AC1 and AC2 increased from 21.75% and 31.25% to 40.5% and 55% from the 4th week to the 8th week respectively. The AC3 and AC4 decreased from 31% and 15.5% to 1.5 %, 3%. Alkaline phosphatase - alkaline phosphatase (APAAP) assay for cell surface antigen showed AC1 fibronectin positive, AC2 CD68 strongly positive, weak AC3 fibronectin, AC4 the upper two monoclonal antibodies and Factor VIII Relevant active antigens are negative. The results of this experiment can be used as a reference value to measure changes in the composition of stromal cells under various experimental conditions.