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目的:探讨丙型肝炎(HC)患者血清中病毒基因水平的消长与病情及预后的关系。方法:采用“ELISA模式”化的HCV-RNA聚合酶链反应杂交定量(PCR-Hyb),检测了19例慢性丙型肝炎患者,α-干扰素治疗前后血清中的HCV-RNA水平结果:在α-干扰素治疗前,8例慢性迁延性肝炎(CPH)患者血清中HCV-RNA平均含量(4.2±1.8)×10~4fg/μl,明显低于11例慢性活动性肝炎(5.9±1.5)×10~4fg/μl,P＜0.05。干扰素治疗CPH中完全反应者占87.5%,明显高于CAH的63.6%,(P＜0.05)7例慢性活动性肝炎(CAH)完全反应者,在疗程结束后还存在着浓度很高的HCV-RNA,且有3例在疗程结束后半年,血清中乃有较高水平的HCV-RNA。结论:PCR-Hyb定量检测HCV-RNA,有助于治疗对象的选择、病情分析、疗效的预测和评价。
Objective: To investigate the relationship between the growth and decline of the viral genes and the prognosis in patients with hepatitis C (HC). Methods: Serum HCV-RNA levels in 19 chronic hepatitis C patients before and after IFN-α treatment were detected by “ELISA-based” PCR-Hyb. The average level of HCV-RNA in serum of 8 chronic CP patients (4.2 ± 1.8) × 10 ~ 4fg / μl before a-interferon treatment was significantly lower than that of 11 chronic active hepatitis (5.9 ± 1.5) × 10 ~ 4fg / μl, P <0.05. Interferon therapy accounted for 87.5% of the total responders in CPH, which was significantly higher than 63.6% of CAH (P <0.05). Seven of 7 patients with chronic active hepatitis (CAH) were completely responders. After treatment, there was still a high concentration of HCV -RNA, and three cases in the course of six months after the end of the serum is higher levels of HCV-RNA. Conclusion: The quantitative detection of HCV-RNA by PCR-Hyb is helpful for the selection of the treatment subjects, the analysis of the disease, the prediction and evaluation of the curative effect.