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目的:探讨人前列腺癌特异性骨转移的细胞遗传学机制。方法:应用比较基因组杂交技术对18例前列腺癌患者进行染色体变异情况的初步分析,确定可能与骨转移密切相关的变异染色体。再应用PCR及微卫星多态性技术重点对10号染色体上的7个微卫星位点进行杂合性缺失(LOH)检测。结果:11例伴有远处骨转移的患者组织样本中,10号染色体的变异率为90.9%(10/11),显著高于其他染色体(P<0.01);进一步分析发现,7个微卫星位点的LOH现象中,骨转移患者的发生率最高,且以D10S1693～D10S587(10q24.2～q25.3)区的LOH发生率最高。结论:10号染色体上D10S1693～D10S587区(10q24.2～q25.3)是前列腺癌骨转移患者中一个高频的LOH区,此区可能与前列腺癌患者远处骨转移的发生密切相关。
Objective: To explore the cytogenetic mechanism of human prostate cancer-specific bone metastasis. Methods: Chromosome aberrations in 18 patients with prostate cancer were analyzed by comparative genomic hybridization to identify the mutation chromosomes that may be closely related to bone metastasis. PCR and microsatellite polymorphism were used to detect the loss of heterozygosity (LOH) at the 7 microsatellite loci on chromosome 10. Results: The mutation rate of chromosome 10 in 10 cases with distant bone metastases was 90.9% (10/11) which was significantly higher than other chromosomes (P <0.01). Further analysis showed that 7 microsatellites Among the LOH phenotypes, the incidence of bone metastasis was the highest, and the incidence of LOH was the highest in D10S1693 ~ D10S587 (10q24.2 ~ q25.3). Conclusion: D10S1693 ~ D10S587 region (10q24.2 ~ q25.3) on chromosome 10 is a high frequency region of LOH in patients with prostate cancer with bone metastasis. This region may be closely related to the distant metastasis of bone cancer in patients with prostate cancer.