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[Objective] The aim was to investigate the technological parameters of the refining process of Icraiin from Epimedium wushanense T. S. Ying by polyamide. [Method] Polyamide chromatogram was adopted to separate and purify. Gradient elution used 10%-40% alcohol, and HPLC was adopted to undertake assaying of the Icraiin. [Result] The content of the Icraiin by adopting the gradient elution with 30% alcohol was 17.22%. Total yield reached 95.97%. [Conclution] The method is simple to operate and has good reproducibility. It can purify and enrich the Icraiin from E. wushanense effectively.
[Objective] The aim was to investigate the technological parameters of the refining process of Icraiin from Epimedium wushanense TS Ying by polyamide. [Method] Polyamide chromatogram was adopted to separate and purify. Gradient elution used 10% -40% alcohol, and HPLC was [Result] The content of the Icraiin by adopting the gradient elution with 30% alcohol was 17.22%. Total yield reached 95.97%. [Result] The method is simple to operate and has good reproducibility. It can purify and enrich the Icraiin from E. wushanense effectively.