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目的检测HPVL1和16/18DNA在喉癌组织中的表达,探讨HPV在喉癌发生中的作用。方法用PCR检测123例喉癌组织及123例喉粘膜上皮组织中HPVL1和16/18DNA。结果在123例喉癌标本中,HPV-L1阳性率为76.42%;HPV-16E6阳性率为54.47%;HPV-16E7阳性率为58.54%;HPV-18E6阳性率为42.28%;HPV-18E7阳性率为48.78%.123例喉粘膜PCR结果表明:低危型HPV的阳性率与喉癌相近;高危型HPV的阳性率虽远远低于喉癌,但随着组织病变程度加重阳性率逐渐升高;HPV-L1的阳性率高于HPV-16E6、E7,随着组织病变程度加重,HPV-L1的检出率逐渐增高。结论 HPV-16感染是重庆地区喉癌的重要发病因素;HPV-16感染后E6/E7片段的保留并持续表达与喉组织的癌变进程密切相关;HPV-16感染会增强喉上皮病变的风险,而HPV-18有协同作用;L1片段适合用于检测HPV感染。
Objective To detect the expression of HPVL1 and 16/18 DNA in laryngeal squamous cell carcinoma and to explore the role of HPV in laryngeal carcinogenesis. Methods The HPVL1 and 16/18 DNA in 123 cases of laryngeal carcinoma and 123 cases of laryngeal mucosal epithelium were detected by PCR. Results The positive rate of HPV-L1 was 76.42% in 123 specimens of laryngeal cancer, the positive rate of HPV-16E6 was 54.47%, the positive rate of HPV-16E7 was 58.54%, the positive rate of HPV-18E6 was 42.28% (48.78%) .123 cases of laryngeal mucosa PCR results showed that: the positive rate of low-risk HPV and laryngeal cancer are similar; high-risk HPV positive rate is much lower than laryngeal cancer, but with the degree of tissue lesions increased the positive rate was gradually increased The positive rate of HPV-L1 was higher than that of HPV-16E6 and E7. The detection rate of HPV-L1 was gradually increased with the increase of histological grade. Conclusion HPV-16 infection is an important risk factor for laryngeal cancer in Chongqing area. The retention and persistence of E6 / E7 fragment after HPV-16 infection is closely related to the carcinogenesis of laryngeal tissue. HPV-16 infection may increase the risk of laryngeal epithelial lesions, While HPV-18 has a synergistic effect; L1 fragment is suitable for detecting HPV infection.