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目的:检测C/EBPα在结肠直肠癌中的表达情况,探讨下调C/EBPα表达对结肠癌细胞增殖、凋亡以及细胞周期的影响。方法:利用RT-PCR和免疫组织化学技术检测C/EBPα在结肠直肠癌组织及相邻正常组织中的表达,分析C/EBPα表达与临床病理特征的关系。用Western印迹法筛选C/EBPα,发现SW620为高表达C/EBPα肠癌细胞株。通过RNA干扰技术下调结肠癌细胞株SW620中C/EBPα表达后,利用CCK8和流式分析技术检测SW620细胞增殖、凋亡和细胞周期的变化。结果:C/EBPα在结肠直肠癌组织中高表达,且与肿瘤浸润深度有关(P<0.05);下调C/EBPα表达抑制SW620细胞株增殖(P<0.05),促进SW620细胞凋亡(P<0.05),并使SW620细胞阻滞于细胞周期的S期。结论:C/EBPα在结肠直肠癌组织中高表达,下调C/EBPα可抑制结肠癌细胞增殖,促进其凋亡,阻滞细胞周期。
OBJECTIVE: To detect the expression of C / EBPα in colorectal cancer and to investigate the effects of down-regulation of C / EBPα on the proliferation, apoptosis and cell cycle of colon cancer cells. Methods: The expression of C / EBPα in colorectal cancer tissues and adjacent normal tissues was detected by RT-PCR and immunohistochemistry. The relationship between C / EBPα expression and clinicopathological features was analyzed. The C / EBPα was screened by Western blotting and SW620 was found to be highly expressed C / EBPα colon cancer cell line. The expression of C / EBPα in human colon cancer cell line SW620 was down-regulated by RNAi technique. The proliferation, apoptosis and cell cycle of SW620 cells were detected by CCK8 and flow cytometry. Results: C / EBPα was highly expressed in colorectal cancer tissues and was related to the depth of tumor invasion (P <0.05). The down-regulation of C / EBPα expression inhibited the proliferation of SW620 cells (P <0.05), and promoted the apoptosis of SW620 cells ), And arrests SW620 cells in the S phase of the cell cycle. Conclusion: C / EBPα is overexpressed in colorectal cancer tissues. Down-regulation of C / EBPα can inhibit the proliferation of colon cancer cells, promote apoptosis and arrest the cell cycle.