用MBP89-101加完全性剂免疫SJL／J小鼠，建立EAE动物模型，取发病动物淋巴结作细胞培养，并用MBPP89-101激活培养3周，其间2次经Ficoll－Hypaque分离纯化激活细胞并用X线照射的脾细胞作为抗原提呈细胞。应用3H－TdR参入法检测细胞对抗原的特异性反应并用PE-抗CD4荧光直接染色和FACS分析细胞表型阳性％，证明建立的CD4＋T细胞林对P89-101呈特异反应。 SJL / J mice were immunized with MBP89-101 plus complete agent to establish an animal model of EAE. Lymph nodes from the diseased animals were used for cell culture and activated with MBPP89-101 for 3 weeks. During the second immunization, cells were isolated and purified by Ficoll-Hypaque, Line-irradiated splenocytes act as antigen presenting cells. The specific reaction of the cells against the antigen was detected by 3H-TdR incorporation method, and the positive cell phenotype was analyzed by PE-anti-CD4 fluorescence staining and FACS. It was proved that the established CD4 + T cell-specific response to P89-101 was specific.