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为探讨微波对燕麦种子处理及其Na_2CO_3胁迫下燕麦苗期蛋白质和核酸代谢的影响,以白燕7号燕麦品种为材料,采用2450 MHz频率下,不同功率及时间的微波对种子进行预处理,比较了不同处理对燕麦幼苗早期代谢和发育的影响,结果表明:低功率(36%)预处理对Na_2CO_3胁迫下单株干物质积累及株高和根长有明显的促进作用,诱导激活了叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)的活性,抑制了蛋白水解酶和核糖核酸酶活性,增加了核酸和蛋白质的积累,降低叶片中游离氨基酸含量,其中,30 s处理组显著优于对照组(p<0.05);中等功率(58%)15 s处理仍具有正向调控作用,但随处理时间的增加抑制作用显现和增强。微波处理燕麦种子,可以在碱性盐逆境下,通过调控苗期氮代谢,促进碳流由光合碳代谢向氮代谢的转变,维持盐害下燕麦幼苗核酸和蛋白质代谢的正常进行,增强燕麦幼苗抵御Na_2CO_3胁迫的能力。
In order to investigate the effect of microwave treatment on the protein and nucleic acid metabolism of oat seedlings under oat seed treatment and Na_2CO_3 stress, the seed of Baiyan No.7 oats was pretreated with microwaves of different power and time under the frequency of 2450 MHz, The effects of different treatments on the early metabolism and development of oat seedlings were compared. The results showed that low-power (36%) pretreatment significantly promoted the accumulation of dry matter and the plant height and root length under Na_2CO_3 stress, Nitrate reductase (NR) and glutamine synthetase (GS), inhibited the activity of proteolytic enzymes and ribonucleases, increased the accumulation of nucleic acids and proteins and decreased the contents of free amino acids in the leaves. Among them, 30 s treatment group (P <0.05). The medium-power (58%) 15-s treatment still had a positive effect, but with the increase of treatment time, the inhibitory effect appeared and enhanced. Microwave treatment of oat seeds can promote the transformation of carbon flux from photosynthetic carbon metabolism to nitrogen metabolism by regulating nitrogen metabolism at seedling stage under alkaline salt stress and maintain the normal progress of nucleic acid and protein metabolism of oat seedlings under salt stress and enhance the growth of oat seedlings Ability to resist Na_2CO_3 stress.