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为促进我国食用菌生产的发展,采用菌种液体深层培养是一个重要途径。我们于1982年4、5、10月做了平菇500ml摇瓶震荡培养,三次均获得了平菇菌丝球,接着做了平菇液体菌丝球的应用研究,一是把深层培养出的平菇菌液菌球作为原种接进固体培养基制作栽培种。二是直接作为栽培种接进固体培养料作生产栽培。前者获得成功,由于接种量不当,后者尚存在一定问题。试验初报如下:
In order to promote the development of edible fungus production in our country, it is an important way to adopt the deep culture of strain liquid. In April, May and October 1982, we made 500 ml shake flask cultivation of Pleurotus ostreatus, obtained Pleurotus ostreatus mycelium balls three times, and then applied the research on the application of Pleurotus ostreatus liquid mycelium ball. First, Pleurotus ostreatus bacterial spore as a seed into the solid medium production of cultivars. Second, direct access as a cultivated species of solid culture material for production and cultivation. The former is successful, due to inoculum is not appropriate, the latter there are still some problems. Preliminary report as follows: