论文部分内容阅读
目的对中药生品芫花(Daphne genkwaSieb.et Zucc.)花蕾中的化学成分进行研究。方法运用硅胶、Sephadex LH-20柱色谱、ODS柱色谱、制备HPLC、重结晶等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从芫花花蕾体积分数95%乙醇提取物中分离得到10个化合物。分别鉴定为8-甲氧基山奈酚(8-methoxykaempferol,1)、柚皮素(naringenin,2)、芫花素(genkwanin,3)、5,4′-二羟基-7,3′-二甲氧基黄酮(velutin,4)、芫花素-5-O-β-D-葡萄糖苷(genkwanin-5-O-β-D-glucoside,5)、芫花素-5-O-β-D-茜黄樱草糖苷(genkwanin-O-β-D-pri-meveroside,6)、(-)-松脂素((-)-pinoresinol,7)、(-)-落叶松脂素((-)-lariciresinol,8)、(-)-双氢芝麻脂素((-)-dihydrosesamin,9)、瑞香烷型二萜酯-7(daphnane-type diterpene ester-7,10)。结论化合物1为首次从瑞香属植物中分离得到,化合物2,9,10为首次从芫花中分离得到。
Objective To study the chemical constituents from the flower buds of Daphne genkwa Sieb.et Zucc. Methods The constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography, ODS column chromatography, preparative HPLC and recrystallization. Their chemical structures were elucidated on the basis of physico-chemical properties and spectral data. Results Ten compounds were isolated from 95% ethanol extract of flower buds of Daphne genkwa. Were identified as 8-methoxykaempferol (1), naringenin (2), genkwanin (3), 5,4’-dihydroxy- 5-O-β-D-glucoside (5), 5-O-β-D-glucoside Genkwanin-O-β-D-pri-meveroside (6), (-) - pinoresinol, -ricricininol, 8), (-) - dihydrosugarone (9), daphnane-type diterpene ester-7,10. Conclusion Compound 1 was isolated from Dahurianum for the first time. Compounds 2, 9 and 10 were isolated from Daphne genkwa for the first time.