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目的通过体内试验,研究草甘膦(glyphosate)对小鼠机体的氧化损伤作用。方法将昆明种清洁级小鼠按体重随机分为低剂量组(312.5 mg/kg)、中剂量组(625 mg/kg)、高剂量组(1250 mg/kg)和阴性对照组(3%淀粉溶液),经口灌胃连续染毒30 d。染毒结束后,采用分光光度法测定小鼠肝、脾、肾中的丙二醛(MDA)、总抗氧化能力(TAC)及血清中的MDA、TAC、晚期氧化蛋白产物(AOPP)的含量。结果各剂量组肝脏、脾脏和肾脏的脏体比与对照组相比,均无显著性差异(P>0.05);TAC含量随剂量增加呈降低趋势,其中肝脏和肾脏组织的高剂量组TAC含量明显低于对照组(P<0.05),其他各剂量组均无显著性差异(P>0.05);MDA含量随剂量增加有增高趋势,但只有肝脏组织中的高剂量组及脾脏组织中的中剂量组MDA含量明显高于对照组(P<0.05);随着染毒剂量的增加,血清中AOPP含量不断增高,中、高剂量组AOPP含量明显高于对照组(P<0.05),呈一定的剂量-反应关系。结论草甘膦能降低小鼠的总抗氧化能力,损伤蛋白质和脂质,造成机体的氧化损伤,导致各种疾病的发生。
Objective To study the effect of glyphosate on oxidative injury in mice through in vivo experiments. Methods Kunming mice were randomly divided into low dose group (312.5 mg / kg), middle dose group (625 mg / kg), high dose group (1250 mg / kg) and negative control group Solution), continuous oral administration of oral 30 d. After the exposure, the contents of malondialdehyde (MDA), total antioxidant capacity (TAC) and MDA, TAC and AOPP in the liver, spleen and kidney were measured by spectrophotometry . Results Compared with the control group, there was no significant difference in the viscera of liver, spleen and kidney in each dose group (P> 0.05). The content of TAC decreased with the increasing of dose, and the content of TAC in the high dose group of liver and kidney (P <0.05), and no significant difference was observed in other dose groups (P> 0.05). The content of MDA increased with the increase of dose, but only in the high dose group of liver tissue and in the middle of spleen tissue The content of AOPP in the serum was higher than that in the control group (P <0.05). The content of AOPP in the serum was increased with the increase of the dose of AOPP. The content of AOPP in the medium and high dose groups was significantly higher than that of the control group (P <0.05) Dose-response relationship. Conclusion Glyphosate can reduce the total antioxidant capacity of mice, damage proteins and lipids, causing oxidative damage to the body, leading to various diseases.