目的:研究亚血红素6肽(DhHP-6)对宫内缺血缺氧胎鼠脑组织自由基和细胞凋亡的影响,探讨DhHP-6对宫内窘迫胎鼠脑组织的保护作用。方法:Wistar雌性大白鼠,于妊娠第19天通过无损伤动脉钳钳夹双侧子宫、卵巢血管20min建立缺血缺氧宫内窘迫模型,将母鼠随机分为A组(假手术组),B组(缺血缺氧组),DhHP-6治疗组,又分为HI前30 min和HI后立即给药组(即C、D组)。此后实验用母鼠均关腹放回原饲养环境中24h后,立即剖宫取胎,每组随机抽取10只胎鼠断头取脑组织制备标本,行HE染色和TUNEL染色法检查,高倍镜下观察胎鼠脑组织病理改变与海马区神经细胞凋亡情况,剩余胎鼠脑组织冻存,制作匀浆后以比色法测定MDA含量。结果:胎鼠死亡率、MDA含量、海马区细胞凋亡数B组与A组比较,差异具有统计学意义(P<0.01);C组、D组与B组比较,差异有统计学意义(P<0.01、P<0.05);D组MDA含量、细胞凋亡数与C组比较差异具有统计学意义(P<0.01)。结论:DhHP-6有抗氧化和较强的氧自由基清除作用,同时减轻脑组织在宫内已加重的细胞凋亡损伤;缺血缺氧性脑损伤前用药比其后用药效果更佳。 Objective: To study the effect of DhHP-6 on the free radical and apoptosis of brain tissue in fetal rats with intrauterine ischemia and hypoxia and to explore the protective effect of DhHP-6 on the brain tissue of intrauterine fetal distress rats. METHODS: Wistar female rats were randomly divided into A group (sham operation group), control group (sham operation group) and control group Group B (ischemia and hypoxia group) and DhHP-6 treatment group were further divided into 30 min before HI and immediately after HI (group C, D). After that, the experimental maternal mice were housed in the original feeding environment for 24 hours, then the cesarean section was withdrawn immediately. Each group of 10 fetuses were randomly selected for decapitation and brain tissue preparation. HE staining and TUNEL staining were performed. Observed under the pathological changes of fetal rat brain tissue and neuronal apoptosis in the hippocampus, the remaining fetal rat brain tissue frozen, homogenized after the determination of MDA content by colorimetry. Results: There were significant differences in fetal death rate, MDA content and apoptosis in hippocampus between group B and group A (P <0.01). There was significant difference between group C and group D and group B P <0.01, P <0.05). The content of MDA and the number of apoptotic cells in group D were significantly different from those in group C (P <0.01). CONCLUSION: DhHP-6 has antioxidant and strong scavenging effects on oxygen free radicals, and at the same time, it attenuates the increased apoptosis of brain tissue in the uterus. DhHP-6 has a better effect than before administration of hypoxic-ischemic brain damage.