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自六十年代中期以来,从植物花粉诱导单倍体植株的工作,取得了很大进展,但从雌配子体诱导出单倍体植株的报道并不多。我们在1978—1979年进行了烟草胚珠离体培养试验,第一年就获得了大量的愈伤组织,和9棵单倍体植株。现将试验的初步结果报道如下。材料和方法采用普通烟草(Speight G-28×Burley21)F_1、(Speight G-28×Ky56)F_1和红花大金元品种为试验材料。选择植株生长正常、花冠比花萼约长1厘米的花蕾里的胚珠(尚未
Since the mid-1960s, great progress has been made in the induction of haploid plants from plant pollen. However, there are not many reports of haploid plants induced from female gametophytes. In 1978-1979, we conducted a tobacco ovule in vitro culture experiment. In the first year, we obtained lots of callus and 9 haploid plants. The preliminary results of the trial are reported below. MATERIALS AND METHODS Tobacco varieties (Speight G-28 × Burley21 F_1, Speight G-28 × Ky56) F_1 and Honghua Daijin were used as experimental materials. Select the plant growth is normal, the corolla is about 1 cm longer than the calyx ovules (not yet