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采用实时荧光定量PCR对两种不同强制通气方式(罐体内嵌式通气和整体式通气)好氧发酵过程中碳氮代谢相关基因表达,包括细菌16S r DNA、β-glucosidase、amo A、nxr B1基因进行了定量分析,并对比研究了发酵过程中理化参数及堆体中碳氮代谢相关基因表达的变化.实验结果表明,在堆肥过程中,罐体内嵌式通气发酵堆体细菌总量大于整体式通气发酵;罐体内嵌式通气与整体式通气发酵中β-glucosidase基因拷贝数分别在堆肥第7 d与第4 d达到最大值,该数值分别是堆肥第1 d的4.0倍和6.2倍,表明发生了强烈的生物降解作用,碳代谢活动剧烈;氨氧化细菌(AOB)在强制通气好氧发酵的高温期含量极少,直至腐熟期才大量积累,导致大量铵态氮转化为氨气造成氮损失;高温对nxr B1基因表达有严重阻碍作用,在腐熟阶段罐体内嵌式通气方式发酵中nxr B1基因优先于整体式通气发酵表达并达到峰值.
Real-time fluorescence quantitative PCR was used to analyze the gene expression of carbon and nitrogen metabolism related to aerobic fermentation in two different forced ventilation modes (bacterial in-line and integral ventilation), including bacterial 16S rDNA, β-glucosidase, amo A, nxr B1 gene were quantitatively analyzed and the changes of the physicochemical parameters and the expression of genes related to carbon and nitrogen metabolism during the fermentation process were compared.The results showed that during the composting process, Was greater than that of monolithic aeration fermentation. The copy number of β-glucosidase gene in in-tank aeration and monolithic aeration fermentation reached the maximum value on the 7th day and the 4th day of compost respectively, which were 4.0 times and 6.2 times, indicating that strong biodegradation occurred and the activity of carbon metabolism was drastic. AOB was extremely low during the high-temperature aerobic fermentation of forced aeration and accumulated only a great amount during the maturity stage, resulting in the conversion of a large amount of ammonium nitrogen into Ammonia caused nitrogen loss; high temperature had a significant impediment to the nxrB1 gene expression, nxrB1 gene expression and peaked during in-cell aeration fermentations in the maturation stage.