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目的:观察各类型乙型肝炎患者血清HBV DNA复制水平及其与临床病情、乙肝血清学诊断标志变化的关系。方法:应用美国Brotronics公司的一种新型定量PCR HBV DNA检测法测定85例乙肝患者血清HBV DNA。利用荧光素标记引物在扩增过程中能量转换的原理对模板DNA进行定量检测,由计算机自动控制和分析。结果:85例乙型肝炎患者HBV DNA检出率94.1％。63例CHB与LC患者中,HBeAg阳性与抗HBe阳性病例均表现较高的HBV DNA复制水平。7例AH与3例SH乙肝血清学诊断标志不典型,但也表现一定HBV DNA水平。血清ALT的异常变化与HBV DNA复制水平无显著相关性。结论:定量HBV DNA PCR法具有很高灵敏度和特异性,定量PCR测定HBV DNA优于斑点杂交法。
Objective: To observe the level of serum HBV DNA replication in patients with various types of hepatitis B and its relationship with clinical conditions, serological markers of hepatitis B changes. Methods: Serum HBV DNA of 85 patients with hepatitis B were detected by a new quantitative PCR HBV DNA assay from Brotronics Company of USA. Fluorescein-labeled primers in the amplification process of energy conversion principle of quantitative detection of template DNA by the computer automatically control and analysis. Results: The detection rate of HBV DNA in 85 patients with hepatitis B was 94.1%. In 63 cases of CHB and LC patients, HBeAg-positive and anti-HBe-positive cases showed higher levels of HBV DNA replication. Seven cases of AH and three cases of hepatitis B serological diagnosis of atypical markers, but also showed a certain level of HBV DNA. There was no significant correlation between abnormal changes of serum ALT and HBV DNA replication level. Conclusion: Quantitative HBV DNA PCR method has high sensitivity and specificity. Quantitative PCR was superior to dot blot hybridization in detecting HBV DNA.