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[目的]对保定苜蓿锌离子跨膜转运蛋白(Zinc transporter,ZnT)进行生物信息学分析。[方法]利用保定苜蓿锌离子跨膜转运蛋白氨基酸序列,应用生物信息学软件预测了该蛋白质的理化性质、分子表面亲/疏水性及二级结构,并利用不同的在线工具对其跨膜区域结构进行了对比预测。[结果]ZnT蛋白是一个整体疏水性蛋白,含有408个氨基酸,理论等电点为5.94;3种不同的跨膜预测工具预测得到相同的结果,即其存在7个可能的跨膜疏水区域,二级结构中α-helix(Hh)占48.04%、Extended strand(Ee)占9.56%、Random coi(lCc)占42.40%。这与其跨膜蛋白的性质相一致。[结论]mZnT属于锌离子跨膜转运蛋白的CDF家族,负责将细胞膜内的Zn2+转运出细胞,从而降低Zn2+的浓度与毒害。
[Objective] The bioinformatics analysis of Zinc transporter (ZnT) in Baoding alfalfa was carried out. [Method] With the amino acid sequence of zinc ion transmembrane transporter of Baoding, the physical and chemical properties, molecular surface pro-hydrophobicity and secondary structure of the protein were predicted by bioinformatics software, and its transmembrane domain The structure was compared predictively. [Result] The ZnT protein was a whole hydrophobic protein containing 408 amino acids with a theoretical isoelectric point of 5.94. Three different transmembrane prediction tools predicted the same result, that is, there were 7 possible transmembrane hydrophobic regions, Secondary structure α-helix (Hh) accounted for 48.04%, Extended strand (Ee) accounted for 9.56%, Random coi (lCc) accounted for 42.40%. This is consistent with the nature of its transmembrane protein. [Conclusion] mZnT belongs to the CDF family of zinc ion transmembrane transporters and is responsible for transporting Zn2 + from the cell membrane out of the cells, thereby reducing the concentration and toxicity of Zn2 +.