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本文通过出生后７天和２４个月两组大鼠晶状体与２０％三硝基甲苯（ＴＮＴ）甘油：水（８：２）混悬液体外培养，培养箱通５％ＣＯ２，恒温３７℃，观察ＴＮＴ对晶状体上皮细胞ＤＮＡ的损伤程度，结果以反映单链ＤＮＡ断裂强度的Ｆ６３０／Ｆ５３０比值表示。ＨＰＬＣ分析发现经体外ＴＮＴ孵育的晶状体内含有ＴＮＴ及其代谢产物；当共同培养９６ｈ后，Ｆ６４０／Ｆ５３０比值随着ＴＮＴ剂量增加而增大，达到４０μＬ（１１．７４μｍｏｌ／Ｌ）时趋向平稳；当用ＴＮＴ浓度为４．４０μｍｏｌ／Ｌ的相同剂量培养时，４８小时后单链ＤＮＡ断裂最为严重Ｆ６３０／Ｆ５３０比值为１．５０，明显高子正常对照组（Ｐ＜０．０１）；幼鼠单链ＤＮＡ断裂程度显著高于老龄鼠（Ｐ＜０．０１）。
In this study, we cultured in vitro with 7% and 24 months postnatal rat lens and 20% trinitrotoluene (TNT) glycerol: water (8: 2) The degree of DNA damage in lens epithelial cells was observed by TNT. The results were expressed as F630 / F530 ratio, which reflects the breaking strength of single-stranded DNA. HPLC analysis showed that TNT and its metabolites were contained in the lens after in vitro TNT incubation. The ratio of F640 / F530 increased with the increase of TNT dosage when the cells were co-cultured for 96h, reaching a steady level of 40μL (11.74μmol / L) When cultured with the same dose of TNT at 4.40 μmol / L, the ratio of F630 / F530 was the highest at 48 hours, the ratio of F630 / F530 was significantly higher than that of the control group (P <0.01) The degree of strand DNA breakage was significantly higher than that of the aged mice (P <0.01).