【摘 要】
：
A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhanc
【机 构】
：
State Key Laboratory of Bioelectronics,Key Laboratory of Green-Packaging and Application of Bionanot
【基金项目】
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教育部跨世纪优秀人才培养计划;国家自然科学基金;Returned Overseas Chinese Scholars, State Education Ministry of China
论文部分内容阅读
A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection.A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
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