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我国是荞麦的起源中心,荞麦资源十分丰富,所以开展荞麦的遗传多样性研究对荞麦遗传资源的保存和开发利用具有重要意义。本研究利用筛选出的17对SSR引物对来自我国12个省的荞麦栽培品种的遗传多样性进行了研究。结果表明,17对引物共扩增出255个条带,平均15.0条,其中多态性条带228条,平均13.4条,多态率达89.4%;UPGMA聚类分析显示,73份材料被分成2组,所有苦荞材料聚在一起形成第一组,甜荞与花荞聚在一起形成第二组,明确显示苦荞与甜荞间的亲缘关系较远;同一个大组内多数品种显示出较近的亲缘关系,遗传基础相对单一。
China is the center of origin of buckwheat, buckwheat is very rich in resources, so the study of genetic diversity of buckwheat for buckwheat genetic resources conservation and development and utilization of great significance. In this study, 17 SSR primers were screened for the genetic diversity of buckwheat cultivars from 12 provinces in China. The results showed that 255 bands were amplified by 17 pairs of primers, with an average of 15.0 bands, of which 228 bands were polymorphic with an average of 13.4 bands and a polymorphism rate of 89.4%. UPGMA clustering analysis showed that 73 bands were divided into 2 groups, all the buckwheat materials gathered together to form the first group, and the buckwheat and the buckwheat gathered together to form the second group, which clearly showed that the relationship between the buckwheat and the buckwheat was far. In the same group, most varieties showed A closer genetic relationship, the genetic basis is relatively simple.