利用发根农杆菌A4和R1601感染北玄参(Scrophularia buergeriana)叶片外植体,诱导产生毛状根,产生的毛状根可在无激素的液体和固体MS培养基上快速生长。rol B基因的PCR检测表明,Ri质粒中的T-DNA片段整合到了北玄参毛状根的基因组中。毛状根在附加0.5 mg·L–1 6-BA及0.1 mg·L–1 NAA的MS固体培养基上形成绿色愈伤组织,之后形成不定芽,并获得再生植株;毛状根在附加0.5 mg·L–1 6-BA及0.02 mg·L–1 NAA的MS固体培养基上培养约15–20天可直接形成不定芽,且不定芽的诱导率达85%。 Hairy roots were induced by Agrobacterium rhizogenes A4 and R1601 infection of leaf explants of Scrophularia buergeriana. The hairy roots produced could rapidly grow on hormone-free liquid and solid MS medium. PCR analysis of the rol B gene showed that the T-DNA fragment in the Ri plasmid was integrated into the genome of the hairy roots of the genus Xerox. Hairy roots formed green callus on MS solid medium supplemented with 0.5 mg · L-1 6-BA and 0.1 mg · L-1 NAA, then formed adventitious buds and obtained regenerated plants; Adventitious buds were formed directly on MS solid medium with mg · L-1 6-BA and 0.02 mg · L-1 NAA for about 15-20 days, and the induction rate of adventitious buds was 85%.