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为探讨血小板源生长因子(PDGF)在胃癌发生发展中的作用。对18例胃癌、36例慢性胃炎和15例对照组,应用快速尿素酶试验和Warthin—starry法检测HP;采用细胞生物活性法测定胃癌、胃炎组织和胃癌细胞株MGc803培养上清液中的PDGF活性。结果HP感染率在胃癌组和胃炎组无显著性差异。胃癌、胃炎组织和胃癌细胞培养上清液中均能测到PDGF活性,胃癌组要明显高于胃炎组。PDGF可明显促进胃癌细胞的体外增殖,且呈剂量依赖关系,抗—PDGF抗体却可明显抑制胃癌细胞的增殖,且呈剂量依赖关系,抗—PDGF抗体却可明显抑制胃癌细胞的增殖。结果提示PDGF的异常表达可能与胃癌的发病机理有关。
To investigate the role of platelet-derived growth factor (PDGF) in the development of gastric cancer. 18 cases of gastric cancer, 36 cases of chronic gastritis and 15 cases of control group, rapid urease test and Warthin-starry method for the detection of HP; cell biological activity of gastric cancer, gastritis and gastric cancer cell line MGc803 culture supernatant of PDGF active. Results HP infection rate in gastric cancer group and gastritis group no significant difference. Gastric cancer, gastritis and gastric cancer cell culture supernatant can be measured PDGF activity, gastric cancer was significantly higher than the gastritis group. PDGF can significantly promote the proliferation of gastric cancer cells in a dose-dependent manner. Anti-PDGF antibody can significantly inhibit the proliferation of gastric cancer cells in a dose-dependent manner. Anti-PDGF antibody can significantly inhibit the proliferation of gastric cancer cells. The results suggest that the abnormal expression of PDGF may be related to the pathogenesis of gastric cancer.