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为探究不同基因型玉米幼苗对锌敏感性差异,以玉米杂交品种天农九(TN9)和信玉9(XY9)为材料,研究不同Zn浓度(0.00、0.01、0.1、1.0、10.0、100μmol·L-1)对叶片光合色素含量、光合作用参数及叶绿素荧光特性的影响。结果表明,玉米最适生长锌浓度为0.1~1μmol·L-1,低锌(0~0.01μmol·L-1)和高锌(10~100μmol·L-1)条件均对不同基因型玉米幼苗的生长产生抑制作用,其中XY9在低锌及高锌胁迫下光合参数及光合色素含量降幅显著,从而导致其植株长势和干物质积累等对锌浓度的动态响应更为强烈,而TN9则对低锌及高锌环境具有较强的适应性。同时,随着锌处理浓度的不断升高,玉米植株中锌含量和积累量均逐渐升高,锌利用率则逐渐降低,其中XY9植株锌积累量低于TN9,而锌利用率则高于TN9;相比于不敏感型品种(TN9),敏感型品种(XY9)对锌的吸收、积累和利用受锌浓度变化的影响更为明显。本研究为植物锌高效利用研究提供理论基础。
In order to explore the differences of zinc sensitivity among different genotypes of maize seedlings, TN9 and X9 were used to study the effects of different concentrations of Zn (0.00,0.01,0.1,1.0,10.0,100μmol·L -1) on photosynthetic pigment content, photosynthesis parameters and chlorophyll fluorescence of leaves. The results showed that the optimum zinc concentration for maize growth was 0.1-1 μmol·L-1, and the low-zinc (0-0.01 μmol·L-1) and high zinc (10-100 μmol·L-1) , And the photosynthetic parameters and photosynthetic pigment content of XY9 decreased significantly under the condition of low zinc and high zinc, which led to the stronger dynamic response to zinc concentration, such as plant growth and dry matter accumulation, while the activity of TN9 was lower Zinc and high zinc environment has strong adaptability. At the same time, with the increasing of zinc concentration, the content and accumulative amount of zinc in corn plants increased gradually and the zinc utilization rate decreased gradually. The accumulative amount of zinc in XY9 plants was lower than that of TN9, but the zinc utilization rate was higher than that of TN9 Compared with insensitive variety (TN9), the sensitivity of XY9 to zinc absorption, accumulation and utilization was more affected by the change of zinc concentration. This study provides a theoretical basis for the research on the efficient use of plant zinc.