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目的 了解山东省艾滋病的流行特征、感染来源及发展规律 ,为制定山东省艾滋病的防治目标和策略提供依据。方法 采用酶联免疫吸附试验 (ELISA)、免疫印迹试验 (WB) ,对高危重点人群血液标本进行人免疫缺陷病毒 (HIV)抗体检测 ,对抗 HIV阳性标本进行个案流行病学调查 ,同时重新采集血液标本核实感染者 ,并从防凝全血中分离单核细胞 (PBMC) ,提取前病毒DNA ,经多聚酶链反应扩增 ,进行基因序列测定和亚型分析。结果 从 1987年到 1999年底 ,全省共检测 80 5 80 1份标本 ,检出HIV抗体阳性 5 2份 ,检出率为 0 .0 6‰ ,其中艾滋病病人 2例 ,均已死亡。对 2 4份感染者的PBMC进行序列测定和亚型分析 ,HIV 1M亚群共有 5种基因亚型 :A、B、B’、C和E ,分布在全省的 7个市地 ,以采供血 (浆 )和性途径传播为主 ,分别占 5 9 6 2 %和 30 76 %。其中 13名有偿供血(浆 )者均为B’(泰国B)亚型 ,10名回国劳工及 1名劳工配偶中有 6名为C亚型 ,2名B亚型 ,A、E亚型各 1名。结论 流行病学资料分析表明 ,大多数感染者流动性很强。山东省HIV感染以采供血(浆 )、性途径传播为主。在性乱人群中已查出HIV感染者 ,其传播流行的可能性很大。应加强对供血 (浆 )、性乱者及回国劳工的监测。
Objective To understand the epidemic characteristics, origin and development of AIDS in Shandong Province and provide the basis for the development of AIDS prevention and control goals and strategies in Shandong Province. Methods Human immunodeficiency virus (HIV) antibody was detected in blood samples of high-risk key population by enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB), and case-based epidemiological investigation was performed on HIV-positive specimens. At the same time, blood samples were collected again Specimens were verified for infection and mononuclear cells (PBMCs) were isolated from anticoagulant whole blood, and proviral DNA was extracted and amplified by polymerase chain reaction for gene sequence analysis and subtype analysis. Results From 1987 to the end of 1999, a total of 80 5 80 1 specimens were detected in the province. 52 positive HIV antibodies were detected with a detection rate of 0.06 ‰, including 2 AIDS patients, all of whom died. A total of 5 genotypes of HIV-1 subunits were sequenced and subtype analyzed in 24 PBMCs of PBMCs: A, B, B ’, C and E, which were distributed in 7 provinces in the province. Blood (plasma) and sexual transmission were the major components, accounting for 59.6% and 30.76% respectively. Among them, 13 paid blood donors (B) were subtype B (Thai B), 6 were C subtype, 2 B subtype, A subtype and E subtype E 1 name. Conclusion Epidemiological data analysis shows that most people with HIV are highly mobile. HIV infection in Shandong Province to take blood (pulp), the main route of sexual transmission. HIV-infected people have been detected in sexual disorders, the spread of the possibility of its popularity. The monitoring of blood supply (pulp), sex workers and returning workers should be strengthened.