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目的 探讨戊四氮致疒间 大鼠脑内海马区S 10 0 β蛋白的变化与癫 疒间 发作持续时间和发作强度之间的关系。方法 应用免疫组化法检测惊厥组、癫疒间 持续状态组和对照组大鼠海马内S 10 0 β蛋白阳性细胞数的变化。结果 惊厥组大鼠海马内S 10 0 β蛋白阳性细胞数于致 疒间 后 12h开始增加 ,2 4h达高峰 ,72h恢复 ,与对照组相比差异有显著性 (P <0 0 1)。癫疒间 持续状态组大鼠海马内S 10 0 β蛋白在 12h、2 4h均有明显增加 ,较惊厥组更为显著。两组相比差异有显著性 (P <0 0 1)。结论 致疒间 大鼠海马内S 10 0 β蛋白阳性细胞数及染色强度与癫疒间 持续时间及发作强度有关 ,提示S 10 0 β蛋白可作为临床上判断癫 疒间 后脑损伤、特别是神经胶质细胞损伤的一种较灵敏而特异的指标。
Objective To investigate the relationship between the change of S 10 0 β protein in hippocampus and the duration of epileptic seizures and the intensity of seizures in rats with pentylenetetrazole. Methods Immunohistochemistry was used to detect the number of S 10 0 β-positive cells in the hippocampus of the convulsion group, the persistent state group of epilepsy and the control group. Results Compared with the control group, the number of S 10 0 β positive cells in the hippocampus of rats in seizure group increased at 12 h, reached the peak at 24 h and recovered at 72 h (P <0.01). The hippocampal S 10 0 β protein in the epilepticus continuous state group increased significantly at 12 and 24 hours, which was more significant than that in the seizure group. There was significant difference between the two groups (P <0.01). Conclusions The number of S 10 0 β-positive cells in the hippocampus and the intensity of the staining in the hippocampus are related to the duration of epilepsy and the intensity of the seizure. It suggests that the S 10 0 β protein can be used as a clinical marker for the diagnosis of post-epileptic brain injury, especially the nerve A more sensitive and specific indicator of glial injury.