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目的观察柚皮素对人支气管上皮细胞趋化因子生成的影响,并研究其可能的机制。方法培养人支气管上皮细胞,分为对照组、瘤坏死因子α(TNF-α)组、柚皮素低剂量组、柚皮素中剂量组和柚皮素高剂量组。柚皮素干预组分别与2.5、5和10μmol/L的柚皮素共孵育2 h后,与TNF-α组一起加入TNF-α进行刺激。24 h后用ELISA方法检测各组细胞上清液中Eotaxin及RANTES的表达,Western blot法检测细胞中IκBα含量;另外在反应30 min时,用EMSA法检测核转录因子κB(NF-κB)的DNA结合活性。结果 TNF-α组的趋化因子水平较对照组明显增高。随着柚皮素干预剂量的增大,趋化因子水平明显降低。柚皮素能抑制IκBα降解,并能降低NF-κB的DNA结合活性。结论柚皮素可能通过抑制NF-κB的活性来影响人支气管上皮炎性趋化因子的生成。
Objective To observe the effect of naringenin on chemokine production in human bronchial epithelial cells and to explore its possible mechanism. Methods Human bronchial epithelial cells were cultured and divided into control group, tumor necrosis factor alpha (TNF-α) group, naringenin low dose group, naringenin middle dose group and naringenin high dose group. Naringenin intervention group were incubated with 2.5, 5 and 10μmol / L naringenin 2h, TNF-α group with TNF-α were stimulated. After 24 h, the expression of Eotaxin and RANTES in the supernatant of each group was detected by ELISA, and the IκBα content in the cells was detected by Western blot. At 30 min after the reaction, the expression of NF-κB DNA binding activity. Results The level of chemokines in TNF-α group was significantly higher than that in control group. As naringenin intervention dose increased, chemokine levels significantly reduced. Naringenin inhibits the degradation of IκBα and decreases the DNA-binding activity of NF-κB. Conclusion Naringenin may affect the production of inflammatory chemokines in human bronchial epithelial cells by inhibiting the activity of NF-κB.