论文部分内容阅读
目的研制石头鱼毒素(neoverrucotoxin,stonefish toxin,neoVTX)的胶体金检测试纸条,建立一种该毒素的快速检测方法。方法采用柠檬酸三钠还原法制备胶体金溶液,以neoVTX马血清抗体用于胶体金标记,并将该抗体固定于硝酸纤维素膜作为捕获抗体,利用双抗体夹心法原理制备胶体金检测试纸条。结果制备的检测试纸条对该毒素检测灵敏度为50 ng/ml,该试纸与牛血清白蛋白、卵清蛋白及水母毒素等蛋白无反应,具有较好的特异性。结论成功研制了石头鱼毒素胶体金检测试纸,该试纸条灵敏度高,特异性好,可用于该毒素的快速检测。
Objective To develop a colloidal gold test strip for neithrucotoxin (stonefish toxin, neoVTX) and to establish a rapid method for the detection of the toxin. Methods The colloidal gold solution was prepared by the method of trisodium citrate reduction. The neoVTX horse serum antibody was used for colloidal gold labeling. The antibody was immobilized on nitrocellulose membrane as capture antibody. The colloidal gold test strip was prepared by double antibody sandwich method Article. Results The prepared test strip showed a sensitivity of 50 ng / ml. The test strip showed good specificity with no protein such as bovine serum albumin, ovalbumin and jellyfish toxin. Conclusion The stonefish toxin colloidal gold test paper has been successfully developed. The test strip has high sensitivity and specificity and can be used for the rapid detection of the toxin.