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按“锤头结构”模型设计、合成,并筛选到了针对活化癌基因T24-ras第十二位密码子G→T点突变的核酶克隆。在镁离子存在条件下,该核酶能选择定点切割T24-ras转录物而对正常C-Ha-ras转录物不起任何破坏作用;在接近生理环境及C-Ha-ras与T24-ras mRNA同时存在条件下,该核酶仍能有效选择性切割突变基因产物。这为利用核酶技术治疗因基因突变引起的机体病变提供了现实的可能性。
According to the “hammer structure” model design, synthesis, and screening for activation of oncogene T24-ras twelfth codon G → T point mutation ribozyme cloning. In the presence of magnesium ions, this ribozyme can be selected to site-cut T24-ras transcripts without any disruption of normal C-Ha-ras transcripts; in a near physiological environment with C-Ha-ras and T24-ras mRNA At the same time, the ribozyme can still effectively and selectively cut the mutant gene product. This provides a realistic possibility for using the ribozyme technology to treat the pathological changes caused by gene mutations.