肺炎双球菌较难在实验室中长期培养保存。作者对45株肺炎双球菌分离物分别接种子鸡蛋培养基和经补充的哥伦比亚培养基，于37℃孵育过夜后，放室温（21℃）或4℃保存。发现所有分离物都能在鸡蛋培养基上室温中存活44天，而在哥伦比亚培养基上则只有半数能存活30天。故作者推荐用 Streptococcus pneumoniae difficult to long-term culture in the laboratory preservation. Forty-five isolates of pneumococcus were inoculated with seed egg medium and supplemented Colombian medium, respectively, and incubated overnight at 37 ° C and then stored at room temperature (21 ° C) or 4 ° C. All isolates were found to survive for 44 days at room temperature in egg medium, whereas only half of Colombian medium survived for 30 days. It is recommended by the author