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目的：研究非小细胞肺癌与ｐ５３基因突变的关系。方法：采用聚合酶链式反应和ＤＮＡ双链直接测序方法对３７例非小细胞肺癌组织标本进行Ｐ５３基因外显子５，６，７，８序列分析。结果：１５例突变，总突变率为４０．５４％。分别为２３７，２４３位密码子突变各１例，２８５位密码子突变２例，２７３位密码子突变的７例，另有４例标本同时存在第２５２和２５４位两个密码子突变为ＴＴＣ，这种突变方式尚未见报道。存在突变的１５例患者中１３例在一年内死亡，无突变２２例中只有１０例患者在一年内死亡，存在ｐ５３突变的肺癌近期死亡率明显升高（Ｐ＜０．０１）。结论：非小细胞肺癌与ｐ５３基因突变密切相关，突变的方式在不同人群中有所不同，ｐ５３基因测序在肺癌诊断和判断预后等方面有一定的参考价值。
Objective: To study the relationship between non-small cell lung cancer and p53 gene mutation. METHODS: The exon 5, 6, 7, 8 sequences of P53 gene were analyzed in 37 non-small cell lung cancer tissues by polymerase chain reaction and DNA double strand direct sequencing. Results: 15 cases of mutations, the total mutation rate was 40.54%. There were one case of 237,243 codon mutations, two cases of 285 codon mutations, seven cases of 273 codon mutations, and another four cases where both codons 252 and 254 were mutated to TTC. This mutation has not been reported. Thirteen of the 15 patients with mutations died within one year. Only 22 of the 22 patients without mutation died within a year. The recent mortality of patients with p53 mutation was significantly higher (P<0.01). Conclusion: Non-small cell lung cancer is closely related to p53 gene mutation. The mutation pattern is different in different populations. The sequencing of p53 gene has certain reference value in the diagnosis and prognosis of lung cancer.