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目的 探讨p5 3基因点突变与人非小细胞肺癌临床病理生理特征的联系。方法 应用聚合酶反应 -单链构象多态性分析方法 (PCR SSCP)检测原发性非小细胞肺癌癌组织的p5 3基因第 5～ 8外显子点突变。结果 40例肺癌组织中 19例 ( 4 7.5 % )有点突变发生 ,8例良性肿瘤组织均无p5 3基因点突变发生。点突变发生与病理分期和淋巴结转移有明显关系 (P <0 .0 2 5 )。结论 p5 3基因点突变在非小细胞肺癌的发生和进展中可能起重要作用。
Objective To investigate the relationship between p5 3 gene mutation and clinicopathological features in human non-small cell lung cancer. Methods Polymerase chain reaction-single strand conformation polymorphism (PCR SSCP) was used to detect the mutation of exon 5 to exon 8 of p5 3 gene in primary non-small cell lung cancer. Results In 40 cases of lung cancer, 19 cases (4. 7.5%) had a little mutation, and no mutation of p5 3 gene was found in 8 cases of benign tumor. There was a significant relationship between point mutation and pathological stage and lymph node metastasis (P <0.05). Conclusion The point mutation of p5 3 gene may play an important role in the occurrence and progression of non-small cell lung cancer.