论文部分内容阅读
目的 探讨Th1/Th2类细胞因子基因在人脑胶质瘤中的分泌及表达情况,以及它们在脑胶质瘤发生及发展中的作用。方法 以IL-2、IFNγ代表Th1类细胞因子,IL-4、IL-6及IL-10代表Th2类细胞因子,采用ELISA法检测脑胶质瘤细胞株培养上清中细胞因子的活性,采用逆转录聚合酶链反应方法(RT-PCR)检测Th1/Th2类细胞因子基因在脑胶质瘤细胞、肿瘤浸润淋巴细胞及细胞株中的表达情况。结果 人脑胶质瘤细胞、肿瘤浸润淋巴细胞及脑胶质瘤细胞株均呈现明显的Th2类细胞因子基因优势表达,而正常人脑组织中则无这种表达趋势。结论 Th2类细胞因子在人脑胶质瘤中的优势表达可能是导致脑胶质瘤患者细胞免疫功能低下的原因之一,有可能在脑胶质瘤的发生及发展中起一定作用。
Objective To investigate the secretion and expression of Th1 / Th2 cytokine genes in human glioma and their roles in the development and progression of glioma. Methods Th1 cytokines, IL-4, IL-6 and IL-10 were taken as representative of Th2 cytokines by IL-2 and IFNγ. The activity of cytokines in the culture supernatant of glioma cell lines was detected by ELISA. The expression of Th1 / Th2 cytokines in glioma cells, tumor infiltrating lymphocytes and cell lines was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of Th2 cytokines was predominant in human glioma cells, tumor infiltrating lymphocytes and glioma cell lines, but not in normal human brain. Conclusion The predominant expression of Th2 cytokines in human glioma may be one of the reasons leading to the poor cellular immune function in glioma patients, which may play a role in the occurrence and development of glioma.