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通过计算机分析转座子Tn917的全序列,详细阐述了其物理图谱、结构功能及其转录调节机制.Tn917的5个ORFs排列在同一条DNA链上,且阅读方向都从左至右.ORF1-3起始点的左侧翼排列有启动子序列和Shine-Dalgarno序列.ORF5(编码转座酶)和ORF4(编码拆分酶)的转录方向是一致的,翻译也紧密偶联在一起.在ORF3和ORF4之间存在1个res位点,与Tn3中的res位点基本同源.翻译衰减的功能与rRNA甲基化酶(由ORF2编码的、erm基因的产物)诱导有关,在这个结构基因的左侧翼有200bp的前导区域编码一个具调控功能的36个氨基酸组成的多肽(由ORF1编码).
The complete sequence of transposon Tn917 was analyzed by computer. The physical map, structural function and transcription regulation mechanism were described in detail. The five ORFs of Tn917 are arranged on the same DNA strand, and the reading directions are from left to right. The left flank of the ORF1-3 start is flanked by a promoter sequence and a Shine-Dalgarno sequence. The transcriptional direction of ORF5 (encoding transposase) and ORF4 (encoding splitting enzyme) is consistent and the translations are also tightly coupled. There is one res site between ORF3 and ORF4, which is substantially homologous to the res site in Tn3. The function of translational attenuation is related to the induction of rRNA methylase (a product of the erm gene encoded by ORF2) with a 200 bp leader on the left flank of this structural gene encoding a 36 amino acid polypeptide with regulatory function Encoded by ORF1).