论文部分内容阅读
[目的]研究转基因抗虫棉Bt毒蛋白含量的时空变化及其土壤降解。[方法]采用ELISA法(酶链免疫法)研究和分析了转Btcry1Ac基因抗虫棉的根、茎和叶片组织在不同发育时期毒蛋白的含量变化及转Bt基因抗虫棉(GK45)和非转基因棉花(新陆早36号)在根际土壤、表层土壤和后茬种植区土壤中Btcry1Ac毒蛋白的年平均含量变化。[结果]BtCry1Ac毒蛋白含量在抗虫棉生长过程中均呈动态下降趋势,而根中下降的速率最快,茎和叶片次之;棉花种植区土壤表层中均检测到Btcry1Ac毒蛋白,且后茬种植区中表层毒蛋白的含量增加,而根际土中含量极低。[结论]Btcry1Ac毒蛋白的含量检测为种植转基因作物的风险评价及转基因作物的土壤生态系统安全性评价提供了科学依据。
[Objective] The research aimed to study the spatiotemporal variation of Bt protein content in transgenic Bt cotton and its soil degradation. [Method] The content of toxic protein in root, stem and leaf tissue of transgenic Btcry1Ac transgenic cotton at different developmental stages was analyzed and analyzed by enzyme-linked immunosorbent assay (ELISA), and the transgenic Btcryl cotton (GK45) Changes of annual average contents of Btcry1Ac toxin in rhizosphere soil, topsoil and stubble planting area of transgenic cotton (Xinluzao 36). [Result] The contents of BtCry1Ac toxic protein decreased dynamically during the growth of BtCry1Ac, while the root descended fastest, followed by the stems and leaves. The Btcry1Ac toxic protein was detected in the soil surface of cotton growing area Crops in the area of toxic protein content increased, while the content of rhizosphere soil is very low. [Conclusion] The detection of Btcry1Ac toxin protein provides a scientific basis for evaluating the risk of planting transgenic crops and evaluation of soil ecosystem safety of transgenic crops.