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应用免疫组化ＡＢＣ法，观察２０例前列腺增生症和３０例前列腺癌组织内细胞外基质成份的分布（ＥＣＭ）。其中包括层粘连蛋白（ＬＮ）、纤维连接蛋白（ＦＮ）、Ⅰ、Ⅲ、Ⅳ型胶原蛋白。结果显示：ＬＮ和Ⅳ型胶原分布在上皮基底膜（ＢＭ）内，ＦＮ在基底膜和非基底膜内均有分布，Ⅰ型和Ⅲ型胶原仅分布在间质内。良性病变时ＬＮ、Ⅳ型胶原和ＦＮ（ＢＭ）呈连续线状完整分布，Ⅰ、Ⅲ型胶原含量少，而ＦＮ含量多。癌组织内ＢＭ有不同程度的缺失。Ⅰ、Ⅲ、Ⅳ型胶原分布呈明显的异质性和ＦＮ含量少。上述资料提示前列腺癌组织中ＢＭ明显缺失。Ⅰ、Ⅲ型和Ⅳ型胶原分布呈明显的异质性，这是肿瘤细胞反应的结果，这种改变可能具有限制癌细胞生长的作用。
Immunohistochemical ABC method was used to observe the distribution of extracellular matrix components (ECM) in 20 cases of benign prostatic hyperplasia and 30 cases of prostatic carcinoma. Including laminin (LN), fibronectin (FN), Ⅰ, Ⅲ, Ⅳ collagen. The results showed that LN and type IV collagen were distributed in epithelial basement membrane (BM), FN was distributed in basement membrane and non-basement membrane, and type I and type III collagen distributed only in interstitial. LN, type Ⅳ collagen and FN (BM) showed a continuous linear distribution in benign lesions, with less type I and type III collagen and more FN content. Cancerous BM have varying degrees of deletion. Type Ⅰ, Ⅲ, Ⅳ collagen showed obvious heterogeneity and less FN content. The data suggest that prostate cancer tissue was significantly missing BM. The distribution of type I, type III and type IV collagen showed significant heterogeneity as a result of tumor cell response, which may have the effect of limiting the growth of cancer cells.