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基于GC-MS代谢组学方法分析四逆汤和缺甘草四逆汤干预后大鼠血浆中的内源性代谢物变化,研究四逆汤中甘草对附子的减毒作用。18只SD大鼠,随机平均分为正常组、四逆汤组和缺甘草四逆汤组。四逆汤组和缺甘草四逆汤组分别灌胃药液(生药质量浓度为0.8 g·m L~(-1)),剂量为0.02 m L·g~(-1),每日1次,连续7 d;正常组给予等体积的生理盐水。末次给药0.5 h,收集血浆样本,应用GC-MS检测。数据进行多元统计分析,得到14个潜在代谢标志物(指认13个),并对其相对含量及代谢途径进行分析。与缺甘草方组相比,四逆汤组对7个代谢标志物进行了回调。通过对这些代谢标志物的生理功能分析,推测四逆汤中甘草可以通过调节方中附子对糖酵解、脂质代谢、三羧酸循环和部分氨基酸等生物代谢途径的影响减低附子之毒性。
The changes of endogenous metabolites in plasma after the intervention of Sini Decoction and Glycyrrhiza Sini Decoction were analyzed by GC-MS metabonomics, and the attenuated effects of licorice on aconite were studied. Eighteen Sprague-Dawley rats were randomly divided into normal group, Sini decoction group and Glycyrrhiza Sini Decoction group. Sini Decoction group and Glycyrrhiza Sini Decoction group were administered orally with a crude drug concentration of 0.8 g · m L -1 at a dose of 0.02 m L · g -1 once daily , For 7 consecutive days; normal group given equal volume of saline. 0.5 h after the last administration, plasma samples were collected and analyzed by GC-MS. Data multivariate statistical analysis, 14 potential metabolites markers (identified 13), and its relative content and metabolic pathways were analyzed. Compared with the group without Glycyrrhiza, Sini Decoction had 7 metabolic markers. Through the analysis of the physiological functions of these metabolic markers, it is speculated that the licorice in Sini Decoction can reduce the toxicity of aconite by regulating the effects of aconite on the metabolism pathway of glycolysis, lipid metabolism, tricarboxylic acid cycle and some amino acids.