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目的:探讨补肾活血方对BPH大鼠前列腺导管系统上皮细胞凋亡的影响及其可能机制。方法:3月龄雄性Wistar大鼠100只,随机分为对照组、去势组、模型组、模型加补肾活血组4组,每组25只。其中模型组、模型加补肾活血组以去势加丙酸睾酮法复制BPH模型。自造模起,模型加补肾活血组给予2.34 g/ml补肾活血方流浸膏灌胃,其余各组予以等体积生理盐水灌胃,共37 d。去势后第38天处死各组大鼠,并留取标本。免疫组化法检测前列腺导管系统转化生长因子-β1(TGF-β1)、α-肌动蛋白分布及阳性细胞数;TUNEL法检测前列腺导管系统上皮细胞凋亡率。结果:与模型组相比,模型加补肾活血组无论前列腺导管近段[(36.42±8.10)%vs(15.28±4.30)%,P<0.01]还是远段[(8.71±2.28)%vs(4.42±2.07)%,P<0.05],TGF-β1阳性细胞百分数均明显升高,但只有近段α-肌动蛋白阳性细胞率高于模型组[(28.14±7.43)%vs(18.28±4.07)%,P<0.01],但仍然低于对照组[(33.57±6.85)%,P<0.05]。与对照组比较,模型组和模型加补肾活血组前列腺腺管远、近段上皮细胞凋亡率均明显下降[远段:17.60±4.86 vs 3.07±1.14(P<0.01)、12.37±2.25(P<0.05);近段:39.42±9.20vs 3.86±1.34(P<0.01)、31.14±5.64(P<0.01)]。与模型组比较,模型加补肾活血组前列腺腺管远、近段上皮细胞凋亡率均明显增高(P<0.01)。结论:补肾活血方可通过上调TGF-β1表达,抑制前列腺导管系统近端的平滑肌数量减少,促进前列腺腺管上皮细胞凋亡,从而有效地抑制了良性前列腺增生。
Objective: To investigate the effect of Bushen Huoxue Recipe on the apoptosis of prostatic ductal epithelial cells in BPH rats and its possible mechanism. Methods: One hundred male Wistar rats of 3 months old were randomly divided into control group, castration group, model group, model plus Bushen Huoxue group, with 25 rats in each group. The model group, model and Bushen Huoxue group were castrated BPH model by castrate plus testosterone propionate. Since modeling, the model plus Bushenhuoxue group was given 2.34 g / ml Bushenhuoxue Fang liquid extract gavage, and the remaining groups were given equal volume of normal saline, a total of 37 d. On the 38th day after the castration, rats in each group were sacrificed and the specimens were collected. Immunohistochemistry was used to detect the distribution of TGF-β1 and α-actin in the prostatic ductal system. TUNEL method was used to detect the apoptosis rate of prostatic ductal epithelial cells. Results: Compared with the model group, no significant difference was found between the model and Bushenhuoxue group in the proximal segment of the prostate (36.42 ± 8.10% vs 15.28 ± 4.30%, P <0.01 or in the distal segment (8.71 ± 2.28)% vs (4.42 ± 2.07)%, P <0.05], and the percentage of TGF-β1 positive cells in the model group were significantly higher than those in the untreated group [(28.14 ± 7.43)% vs (18.28 ± 4.07) %, P <0.01], but still lower than the control group [(33.57 ± 6.85)%, P <0.05]. Compared with the control group, the apoptotic rates of the distal and proximal segments of the prostate gland in the model group and the model group with Bushen Huoxue were significantly decreased (remote segment: 17.60 ± 4.86 vs 3.07 ± 1.14 (P <0.01), 12.37 ± 2.25 (P <0.05); the proximal segment: 39.42 ± 9.20vs 3.86 ± 1.34 (P <0.01), 31.14 ± 5.64 (P <0.01)]. Compared with the model group, the apoptosis rate of the proximal and distal epithelial cells in the models plus Bushen Huoxue group were significantly increased (P <0.01). Conclusion: Bushen Huoxue Recipe can inhibit the proliferation of prostatic duct epithelial cells by up-regulating the expression of TGF-β1, inhibiting the decrease of smooth muscle in the proximal part of the prostatic ductal system, and effectively inhibiting the proliferation of benign prostatic hyperplasia.