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本文报道了测定细胞内游离[Ca~(2+)的新荧光试剂Fura-4,Fura-5的合成方法。研究了不同取代基对缩合反应的影响及取代基的结构对荧光性能的影响。
In this paper, we report the synthesis of Fura-4 and Fura-5, a new fluorescent reagent for the determination of intracellular [Ca 2+]. The effects of different substituents on the condensation reaction and the influence of the substituent structure on the fluorescence properties were investigated.