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目的:通过观察中药活血消异方对子宫内膜异位症妊娠功能低下模型小鼠着床窗口期子宫内膜HOXA10基因表达量的影响,探讨子宫内膜异位症对子宫内膜容受性的影响机制以及活血消异方对子宫内膜异位症相关不孕症子宫内膜容受性改善的作用机理。方法:建立子宫内膜异位症妊娠功能低下的小鼠模型,随机分为模型组、中药组及假手术组,采用RT-PCR法检测各组小鼠种植窗口期子宫内膜HOXA10的表达水平。结果:假手术组种植窗口期子宫内膜HOXA10mRNA表达量高于中药组及模型组,差异有统计学意义(P<0.05);而中药组种植窗口期子宫内膜HOXA10mRNA表达量高于模型组,差异有统计学意义(P<0.05)。结论:子宫内膜异位症可导致小鼠种植窗口期HOXA10mRNA表达下调,进而降低其子宫内膜容受性。而中药活血消异方可上调小鼠子宫内膜HOXA10mRNA的表达,改善着床窗口期子宫内膜容受性。
Objective: To observe the effect of Huoxuexianjie on endometrial HOXA10 gene expression in implantation window endometrium in endometriosis model of pregnancy, and to explore the effect of endometriosis on endometrial receptivity And the mechanism of Huoxuexianfang on the improvement of endometrial receptivity in infertility related to endometriosis. Methods: The mouse model of pregnancy with endometriosis was established and randomly divided into model group, traditional Chinese medicine group and sham operation group. The expression level of HOXA10 in the endometrium of the mice in each group was detected by RT-PCR . Results: The expression of HOXA10mRNA in the endometrium of the sham operation group was higher than that of the traditional Chinese medicine group and the model group (P <0.05), while the expression level of HOXA10 mRNA in the endometrium of the Chinese herbal medicine group was higher than that of the model group The difference was statistically significant (P <0.05). Conclusion: Endometriosis can lead to the down-regulation of HOXA10mRNA expression in the window of mice implantation and thus reduce the endometrial receptivity. The traditional Chinese medicine Huoxuexiao Fang can increase the expression of HOXA10 mRNA in mouse endometrium and improve endometrial receptivity in implantation window.