论文部分内容阅读
目的：研究酪氨酸激酶是否参与α１Ａ肾上腺素受体引起血管平滑肌收缩的信号传导．方法：灌流大鼠后肢血管床标本，观察酪氨酸激酶抑制剂对去甲肾上腺素（ＮＥ）引起收缩反应的影响．结果：酪氨酸激酶抑制剂ｔｙｒｐｈｏｓｔｉｎ和ｇｅｎｉｓｔｅｉｎ均显著抑制ＮＥ引起的收缩反应，但对ＫＣｌ引起的收缩反应无影响；酪氨酸磷酸酶抑制剂Ｎａ３ＶＯ４显著加强ＮＥ引起的收缩反应；ｔｙｒｐｈｏｓｔｉｎ和ｇｅｎｉｓｔｅｉｎ对蛋白激酶Ｃ激动剂ｐｈｏｒｂｏｌ１２ｍｙｒｉｓｔａｔｅ１３ａｃｅｔａｔｅ引起的收缩反应均无影响，但均抑制Ｇ蛋白激动剂ＮａＦ引起的收缩反应．结论：Ｔｙｒｐｈｏｓｔｉｎ和ｇｅｎｉｓｔｅｉｎ敏感的酪氨酸激酶参与α１Ａ肾上腺素受体介导的大鼠后肢血管床收缩反应
OBJECTIVE: To investigate whether tyrosine kinase is involved in the signaling of α1A-adrenergic receptor-induced vascular smooth muscle contraction. Methods: Rat vascular hindbrain specimens were perfused to observe the effects of tyrosine kinase inhibitors on norepinephrine (NE) -induced contractile responses. Results: The tyrosine kinase inhibitors tyrphostin and genistein significantly inhibited the contractile response induced by NE, but had no effect on the contractile response induced by KCl. The tyrosine phosphatase inhibitor Na3VO4 significantly enhanced the contractile response induced by NE. The tyrphostin and genistein Protein kinase C agonist phorbol12 myristate13 acetate induced contraction reaction had no effect, but inhibit the G protein agonist NaF induced contraction. CONCLUSION: Tyrphostin and genistein-sensitive tyrosine kinases are involved in α1A-adrenergic receptor-mediated vasoconstriction in rat hindlimbs