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This experiment was conducted to investigate the effect of serum antibody of peptide tyrosine tyrosine (PYY) on activities of digestive enzymes in rats.Sixty Sprague-Dawley (SD) rats weighing 87.77 ± 1.61g were randomly assigned into four treatment groups, with five replicates in each group and three rats for each replicate.For the period of 21 days, four groups were injected PYY antiserum at the dose of 0 (control), 50, 100, or 200 μL respectively.The activity of amylases and lipases in the pancreas and duodenum as well as gastric pepsin was measured at the end of the study.The results showed that the activity of lipases in the pancreas and duodenum increased with the increasing dose of PYY antiserum, but the activity of pancreatic lipases was not significantly different (P > 0.05) within the treated groups.The lipase activity in the duodenum showed a significant increase (P < 0.05) in the groups treated with 100 or 200 μL of PYY antiserum compared with the control group.The amylase activity in the pancreas and duodenum increased with the increasing dose of PYY antiserum, and the group treated with 200 μL of PYY antiserum was significantly higher (P < 0.05) than the other groups.The amylase activity in the duodenum showed no significant difference (P > 0.05) among groups, but the group treated with 200 μL of PYY antiserum showed the highest activity.In conclusion, the injection of PYY antiserum improved nutrition utilization rate and activity of digestive enzymes in a dose dependent manner in rats.