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目的探讨莱菔硫烷(sulforaphane,SFN)对汞所致肝脏氧化应激的拮抗作用。方法实验用Wistar大鼠60只,按体重随机分成6组:对照组,DMSO对照组,低、中、高剂量染汞组、SFN预处理组。对照组和染汞组皮下注射生理盐水,DMSO对照组皮下注射DMSO,SFN预处理组皮下注射2 mg/kg SFN;2 h后,对照组及DMSO对照组腹腔注射生理盐水,低、中、高剂量染汞组分别腹腔注射0.6、1.2、2.4 mg/kg Hg Cl2,SFN预处理组腹腔注射2.4 mg/kg Hg Cl2。注射容量为5 ml/kg。连续预处理与染毒3 d。于最后一次染汞24 h后,每组取6只大鼠,麻醉后处死,冰浴下切取肝组织,测定肝Hg含量、还原型谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GPx)活力及丙二醛(MDA)含量。每组其余4只大鼠,制备肝脏单细胞悬液,测定肝细胞活性氧簇(ROS)及早期细胞凋亡率。结果与对照组比较,随染汞各组染Hg剂量的升高,肝组织Hg、GSH、MDA含量、肝细胞ROS水平及早期细胞凋亡率逐渐升高,SOD及GPx活力逐渐降低,呈现剂量-效应关系(P<0.01)。2.4 mg/kg Hg Cl2组肝组织Hg、GSH、MDA含量、肝细胞ROS水平及早期细胞凋亡率显著升高,SOD及GPx活力显著降低,差异有统计学意义(P<0.05或P<0.01)。SFN预处理组肝组织Hg含量与2.4 mg/kg Hg Cl2组相比未见明显差异(P>0.05),GSH、MDA含量、ROS水平及早期细胞凋亡率显著下降,SOD及GPx活力显著升高,差异有统计学意义(P<0.05或P<0.01)。结论 SFN对汞所致肝脏氧化应激具有一定程度的拮抗作用。
Objective To investigate the antagonistic effect of sulforaphane (SFN) on liver oxidative stress induced by mercury. Methods Sixty Wistar rats were randomly divided into six groups according to body weight: control group, DMSO control group, low, medium and high dose of mercury stain group and SFN pretreatment group. The control group and the mercury-exposed group were injected subcutaneously with normal saline. The DMSO control group was subcutaneously injected with DMSO and SFN pretreatment group with 2 mg / kg SFN. After 2 h, the control group and the DMSO control group were injected with normal saline, low, medium and high Hg Cl2 was intraperitoneally injected with 0.6, 1.2 and 2.4 mg / kg HgCl2, while intraperitoneal injection of 2.4 mg / kg Hg Cl2 was administered to SFN pretreatment group. Injection capacity of 5 ml / kg. Continuous pretreatment and exposure 3 d. After the last 24 h exposure to mercury, 6 rats in each group were killed after anesthesia, and the liver tissue was cut under ice bath to determine the content of Hg, the content of reduced glutathione (GSH), the activity of superoxide dismutase SOD, GPx and malondialdehyde (MDA) were measured. The remaining four rats in each group were used to prepare single cell suspension of liver and the activity of reactive oxygen species (ROS) and early apoptosis of hepatocytes were measured. Results Compared with the control group, the Hg, GSH and MDA contents, the level of ROS in hepatocytes and the rate of early apoptotic cells increased gradually with the increase of Hg dosage in each group, and the activities of SOD and GPx decreased gradually - effect relationship (P <0.01). The contents of Hg, GSH and MDA, the level of ROS in hepatocytes and the rate of early apoptosis in Hg Cl2 group were significantly increased, the activities of SOD and GPx were significantly decreased in 2.4 mg / kg Hg Cl2 group (P <0.05 or P <0.01) ). Compared with 2.4 mg / kg Hg Cl2 group, the content of Hg in liver tissue of SFN pretreatment group had no significant difference (P> 0.05), the content of GSH, MDA, the level of ROS and the rate of early apoptosis were significantly decreased, while the activities of SOD and GPx were significantly increased High, the difference was statistically significant (P <0.05 or P <0.01). Conclusion SFN can antagonize liver oxidative stress induced by mercury to some extent.