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Cardiac ischemia/reperfusion(I/R) injury is a critical condition,often associated with high morbidity and mortality.The cardioprotective effect of grape seed proanthocyanidin extracts(GSPE) against oxidant injury during I/R has been described in previous studies.However,the underlying molecular mechanisms have not been fully elucidated.This study investigated the effect of GSPE on reperfusion arrhythmias especially ventricular tachycardia(VT) and ventricular fibrillation(VF),the lactic acid accumulation and the ultrastructure of ischemic cardiomyocytes as well as the global changes of mitochondria proteins in in vivo rat heart model against I/R injury.GSPE significantly reduced the incidence of VF and VT,lessened the lactic acid accumulation and attenuated the ultrastructure damage.Twenty differential proteins related to cardiac protection were revealed by isobaric tag for relative and absolute quantitation(iTRAQ) profiling.These proteins were mainly involved in energy metabolism.Besides,monoamine oxidase A(MAOA) was also identified.The differential expression of several proteins was validated by Western blot.Our study offered important information on the mechanism of GSPE treatment in ischemic heart disease.
Cardiac ischemia / reperfusion (I / R) injury is a critical condition, often associated with high morbidity and mortality. The cardioprotective effect of grape seed proanthocyanidin extracts (GSPE) against oxidant injury during I / R has been described in previous studies. However, the underlying molecular mechanisms have not been fully elucidated. This study investigated the effect of GSPE on reperfusion arrhythmias especially ventricular tachycardia (VT) and ventricular fibrillation (VF), the lactic acid accumulation and the ultrastructure of ischemic cardiomyocytes as well as the global changes of mitochondria proteins in vivo rat heart model against I / R injury. GSPE significantly reduced the incidence of VF and VT, lessened the lactic acid accumulation and attenuated the ultrastructure damage. Twenty differential proteins related to cardiac protection were revealed by isobaric tag for relative and absolute quantitation (iTRAQ) profiling.These proteins were mainly involved in energy metabolism.Besid es, monoamine oxidase A (MAOA) was also identified. Differential expression of several proteins was validated by Western blot. Our study offers important information on the mechanism of GSPE treatment in ischemic heart disease.