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目的:观察CD151重组腺相关病毒转染心肌梗死小型猪模型对缺血心肌血流灌注的影响,以明确CD151在体内血运重建中的作用。方法:结扎小型猪左前降支建立心肌梗死动物模型。包装CD151、antiCD151和GFP重组腺相关病毒,分点注射至梗死心肌及周围心肌进行基因转染。8周后13N-NH3PET评价心肌血流灌注。结果:CD151基因转染促进心肌组织局部CD151表达增高,明显促进缺血心肌血流灌注。rAAV-CD151组灌注图像心肌缺血总分值为10.82±2.36,明显小于rAAV-GFP组19.33±1.67(P<0.01),而rAAV-an-tiCD151组缺损总分值25.18±2.73,明显大于rAAV-GFP组和rAAV-CD151组(P<0.01)。结论:CD151在体内具有明显的促血管生成作用,能明显促进心肌梗死后血运重建并增加缺血心肌血流灌注。
OBJECTIVE: To observe the effect of CD151 recombinant adeno-associated virus (AAV) on myocardial perfusion in myocardial infarction (MCI) -induced mini-pigs in order to clarify the role of CD151 in revascularization in vivo. Methods: Animal models of myocardial infarction were established by ligation of the left anterior descending branch of miniature pigs. CD151, antiCD151 and GFP recombinant adeno-associated virus were packaged and injected into infarcted myocardium and surrounding myocardium for gene transfection. 13N-NH3PET evaluated myocardial perfusion after 8 weeks. Results: CD151 gene transfection enhanced the local expression of CD151 in myocardium and significantly promoted the perfusion of ischemic myocardium. The total score of myocardial ischemia in rAAV-CD151 group was 10.82 ± 2.36, which was significantly lower than that in rAAV-GFP group (19.33 ± 1.67, P <0.01), while rAAV-an-tiCD151 group was 25.18 ± 2.73, significantly higher than rAAV -GFP group and rAAV-CD151 group (P <0.01). Conclusion: CD151 has obvious angiogenic effect in vivo, which can significantly promote the revascularization after myocardial infarction and increase the perfusion of ischemic myocardium.