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本研究旨在研发经济、高效的人高致病性禽流感病毒H5N1实验疫苗并优化免疫方案。利用本实验室前期构建的含有H5N1(安徽株)结构基因的多个单顺反子(HAop和NAop)和双顺反子(HAop/M2和NAop/M1)DNA疫苗及重组痘苗病毒(天坛株)疫苗,采用不同方案(单独或联合)免疫BALB/c小鼠,初步分析了抗原特异性体液免疫(HA血凝抑制抗体,NA特异性抗体,中和抗体及M1与M2特异性抗体)和细胞免疫应答(IFN-γELIS-pot)的特点。结果表明:DNA疫苗与重组痘苗病毒(天坛株)疫苗联合免疫可以激发较强的多个抗原特异的免疫应答,尤其是体液免疫应答,明显优于DNA疫苗或重组痘苗病毒(天坛株)疫苗单独免疫;联合免疫方案中DNA疫苗初免所激发的HA与NA特异的体液免疫应答强于重组痘苗病毒(天坛株)疫苗初免,然而M1与M2特异的体液免疫应答则反之。本研究为新型H5N1疫苗的研发及免疫方案的优化奠定了基础。
The aim of this study is to develop an economical and efficient human H5N1 vaccine against human avian influenza virus and to optimize its immunization strategy. Multiple monocistronic (HAop and NAop) and bicistronic (HAop / M2 and NAop / M1) DNA vaccines containing recombinant H5N1 (Anhui strain) and recombinant vaccinia virus ) BALB / c mice were immunized with different regimens (alone or in combination), and antigen-specific humoral immunity (HA hemagglutination inhibition antibody, NA specific antibody, neutralizing antibody and M1 and M2 specific antibodies) and Features of the cellular immune response (IFN-γELIS-pot). The results showed that the combination of DNA vaccine and recombinant vaccinia virus (Tiantan) vaccine could stimulate strong multiple antigen-specific immune responses, especially the humoral immune response, which was significantly better than that of DNA vaccine or recombinant vaccinia virus Immunization; HA and NA-specific humoral immune responses induced by DNA vaccine priming in combination regimens were stronger than those of recombinant vaccinia virus (Tiantan) vaccines, however M1 and M2 specific humoral immune responses were reversed. This study laid the foundation for the development of new H5N1 vaccine and the optimization of immunization programs.