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目的探讨褪黑素(MT)对Balb/c小鼠胰腺癌皮下移植瘤的治疗作用及对其自然杀伤(NK)细胞活性的影响。方法在Balb/c小鼠皮下接种胰腺癌SW1990细胞建立荷瘤鼠模型,每组分别用生理盐水、MT低剂量(10mg·㎏-1·d-1)、MT高剂量(20mg·㎏-1·d-1)干预,定期测量肿瘤大小,绘制肿瘤生长曲线,称瘤质量,并用MTT法检测小鼠脾脏(NK)细胞活性。结果与对照组肿瘤体积[(1.476±0.075)cm3]比较,MT低剂量组[(0.998±0.112)cm3]、MT高剂量组[(0.756±0.128)cm3]肿瘤体积显著减小(P<0.01),并且MT高剂量组肿瘤体积明显小于低剂量组(P<0.05)。与对照组肿瘤质量[(1.537±0.106])g]比较,MT低剂量组[(0.898±0.125])g]、MT高剂量组[(0.636±0.081)g]瘤质量显著减轻(P<0.01),并且MT高剂量组瘤质量明显轻于低剂量组(P<0.05)。与对照组NK细胞的杀伤活性[(18.07±1.23)%]相比,MT低剂量组[(44.27±3.19)%]、MT高剂量组[(45.16±3.20)%]NK细胞的杀伤活性显著增强(P<0.01),MT低剂量组与MT高剂量组之间无明显差异(P>0.05)。结论 MT能抑制小鼠皮下移植性胰腺癌的生长,增强荷瘤鼠的免疫功能。
Objective To investigate the therapeutic effect of melatonin (MT) on subcutaneously transplanted pancreatic cancer in Balb / c mice and its effect on natural killer (NK) cell activity. Methods Balb / c mice were subcutaneously inoculated with pancreatic cancer SW1990 cells to establish tumor-bearing mice model. The rats in each group were treated with normal saline, MT low dose (10 mg · ㎏-1 · d-1), MT high dose (20 mg · ㎏ -1 · D-1) intervention, the tumor size was measured regularly, the tumor growth curve was plotted, and the tumor mass was measured. The activity of spleen (NK) cells was detected by MTT assay. Results Compared with the control group, the volume of tumor ([(0.756 ± 0.128) cm3] was significantly decreased in the MT low dose group [(0.998 ± 0.112) cm3] and the MT high dose group ), And the tumor volume of MT high dose group was significantly less than that of low dose group (P <0.05). Compared with the control group, the tumor mass in the high-dose MT group [(0.636 ± 0.081) g] was significantly decreased compared with the control group [(1.537 ± 0.106) ), And the tumor mass of MT high dose group was obviously lighter than that of low dose group (P <0.05). The killing activity of NK cells in MT low dose group [(44.27 ± 3.19)%] and MT high dose group (45.16 ± 3.20)%] was significantly higher than that in control group [(18.07 ± 1.23)%] (P <0.01). There was no significant difference between MT low dose group and MT high dose group (P> 0.05). Conclusion MT can inhibit the growth of subcutaneously transplanted pancreatic cancer in mice and enhance the immune function of tumor-bearing mice.