目的:观察罗格列酮对2型糖尿病大鼠心肌细胞病理改变的作用。方法:实验于2003-01/2003-10在河北医科大学第二医院动物室,河北医科大学电镜室、组胚教研室完成。选择健康8周龄SD雌性大鼠40只。随机选用10只大鼠为对照组:普通饲料喂养。12周时每日给予同实验组等体积的高压消毒水灌胃。给予其余30只大鼠高脂高糖饲料喂养。6周时,腹腔注射小剂量链脲佐菌素(30mg/kg,溶于0.1mol/L枸橼酸缓冲液中,pH4.4)。8周时,24只空腹血糖≥7.8mmol/L(造模成功)。12周时将24只大鼠随机分为糖尿病组和罗格列酮组各12只。罗格列酮组:每日给予罗格列酮1.42mg/(kg·d)灌胃治疗。糖尿病组:每日给予同罗格列酮组等体积的高压消毒水灌胃。24周麻醉大鼠,取出心脏,然后取部分左心室组织置于中性甲醛固定,脱水,石蜡包埋,常规切片,进行苏木精-伊红染色,光镜下观察心肌细胞病理变化,另取部分心室组织透射电镜下观察心肌细胞超微结构变化。结果:①因造模失败脱失6只,最终进入结果分析34只。②光镜可见对照组大鼠心肌细胞排列整齐,致密,结构清晰,细胞核呈卵圆形。糖尿病组大鼠心肌细胞排列紊乱,扭曲,间质有免疫细胞聚集。罗格列酮组心肌细胞排列趋于整齐,结构接近正常。③电镜可见对照组大鼠心肌细胞排列整齐,密集,细胞连接清晰。细胞核呈卵圆形,胞浆内可见大量的结构正常的线粒体和糖原。糖尿病组大鼠肌纤维排列紊乱、稀疏,线粒体肿胀,数量减少,糖原减少。罗格列酮组心肌细胞的结构基本接近正常。结论:罗格列酮可以减轻糖尿病大鼠心肌细胞的病理改变,延缓糖尿病心肌病变的发展。 Objective: To observe the effect of rosiglitazone on the pathological changes of cardiomyocytes in type 2 diabetic rats. Methods: The experiment was performed in the animal room of the Second Hospital of Hebei Medical University, the electron microscope room of Hebei Medical University from 2003-01 / 2003-10, and the embryo of the embryos. Forty female SD rats aged 8 weeks were selected. Ten rats were randomly selected as the control group: normal feed. At 12 weeks, the same experimental group was given the same volume of autoclaved water. The remaining 30 rats were fed high-fat, high-sugar diet. At 6 weeks, a small dose of streptozotocin (30 mg / kg dissolved in 0.1 mol / L citrate buffer, pH 4.4) was injected intraperitoneally. At 8 weeks, 24 fasting blood glucose ≥ 7.8mmol / L (modeling success). At week 12, 24 rats were randomly divided into diabetic group and rosiglitazone group, each with 12 rats. Rosiglitazone group: daily rosiglitazone 1.42mg / (kg · d) gavage treatment. Diabetic group: given daily with the same volume of high-pressure disinfection water rosiglitazone gavage. The rats were anesthetized 24 weeks later, the heart was removed, and then some of the left ventricular tissues were fixed in neutral formaldehyde, dehydrated, embedded in paraffin and stained by hematoxylin-eosin. The pathological changes of cardiomyocytes were observed under light microscope. Take part of the ventricular tissue under transmission electron microscope to observe the changes of myocardial ultrastructure. Results: ① Six animals were lost due to failure of modeling and 34 were finally entered into the result analysis. ② The light microscope shows that the control rat myocardial cells arranged in neat, dense, clear structure, the nucleus was oval. Myocardial cells in diabetic rats arranged disorder, distorted, interstitial immune cells gathered. Cardiomyocytes in rosiglitazone group tended to be neat and the structure was close to normal. ③ Electron microscopy showed that the control rats were arranged in order, and the cells were well connected. The nucleus was oval, a large number of structurally normal mitochondria and glycogen were seen in the cytoplasm. Diabetic rats muscle fibers arranged in disorder, sparse, mitochondria swelling, the number decreased, reduced glycogen. The structure of cardiomyocytes in rosiglitazone group was almost normal. Conclusion: Rosiglitazone can reduce the pathological changes of diabetic rat myocardial cells and delay the development of diabetic cardiomyopathy.