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【目的】定位大豆二粒荚长、宽QTL,并分析QTL间的上位效应和与环境(QTL-by-environment,QE)的互作效应。【方法】利用Charleston×东农594重组自交系及其F2:14—F2:18代的重组自交系的147个株系为试验材料,164个SSR引物经亲本筛选后用于群体扩增构建的SSR遗传图谱,利用混合区间作图法,对2006—2010年连续5年一个地点的大豆二粒荚长、宽进行QTL定位,并作加性效应、加性×加性上位互作效应及环境互作效应分析【。结果】检测到8对有加性效应的二粒荚长QTL,加性效应的总贡献率27.2%,与环境互作总贡献率达到10.19%;6对有加性效应的二粒荚宽QTL,加性效应的总贡献率16.27%,与环境互作总贡献率达到12.18%。9对影响二粒荚长的加性×加性上位互作效应的QTL,可解释该性状总变异的9.02%;8对影响二粒荚宽的加性×加性上位互作效应的QTL,可解释该性状总变异的8.81%。【结论】上位效应和环境效应在二粒荚长、宽性状的遗传中起了重要作用,因此,在分子标记辅助育种中应该考虑对效应起主要作用的QTL和上位性QTL,又要考虑微效多基因的聚合。
【Objective】 The objective of this study was to locate the long and wide QTLs of two pods in soybean and to analyze the interaction effect between epistatic effects and QTL-by-environment (QE). 【Method】 147 lines of recombinant inbred lines of Charleston × Dongnong 594 and their F2: 14-F2: 18 generation inbred lines were used as experimental materials, and 164 SSR primers were screened by their parents for population amplification The constructed SSR genetic map was used to map the QTLs of length and breadth of two-year-old Soybean from 2006 to 2010 for five consecutive years from 2006 to 2010, with additive effects, additive × additive epistatic effects And environmental interaction effect analysis [. Results Eight pairs of long QTLs with additive effect were detected. The total contribution rate of additive effect was 27.2% and the total contribution rate to environmental interaction was 10.19%. Six pairs of wide pod QTLs with additive effect , The total contribution rate of the additive effect is 16.27%, and the total contribution rate to the environment interaction reaches 12.18%. 9 QTLs that affect the additive × additive superiority interaction effect of two pods, explained 9.02% of the total variation of the traits; 8 QTLs affecting the additive × additive superiority interaction effect of two grain pods, Can explain the total variation of 8.81% of the traits. 【Conclusion】 The epistatic and environmental effects played an important role in the inheritance of long and broad traits of two pods. Therefore, QTLs and epistatic QTLs that play a major role in the effects should be considered in molecular marker-assisted breeding. Multipolymerization of genes.