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目的:研究虎杖苷(polydatin)对肝脏药物代谢酶的影响。方法:小鼠灌服虎杖苷240、120和60mg/kg,给药7天,测定肝微粒体细胞色素P450、b5含量和NADPH细胞色素c还原酶、尿苷二磷酸葡萄糖醛酸转移酶(UDPGT)和谷胱甘肽-S-转移酶(GST)活性。结果:小鼠连续多次灌服虎杖苷后可显著提高其肝脏蛋白质含量,肝指数、肝微粒体细胞色素P450、b5含量和NADPH细胞色素c还原酶活性未见明显变化,肝GST和UDPGT活性显著下降。结论:虎杖苷对小鼠肝微粒体Ⅰ相酶没有明显作用,但抑制II相酶UDPGT和GST。
Objective: To study the effect of polydatin on hepatic drug metabolism enzymes. METHODS: Mice were orally administered with polydatin at 240, 120 and 60 mg/kg for 7 days to determine the levels of cytochrome P450 and b5 in liver microsomes and NADPH cytochrome c reductase and uridine diphosphate glucuronyltransferase (UDPGT). ) and glutathione-S-transferase (GST) activity. RESULTS: After continuous administration of polydatin for several times, the liver protein content increased significantly. Liver index, liver microsomal cytochrome P450, b5 content, and NADPH cytochrome c reductase activity did not change significantly. Liver GST and UDPGT activity Decreased significantly. CONCLUSION: Polydatin has no significant effect on mouse liver microsomal phase I enzymes but inhibits phase II enzymes UDPGT and GST.