论文部分内容阅读
目的:观察丹参酮ⅡA磺酸钠(sodium tanshinone Ⅱ A sulfonate,STS)对百草枯中毒大鼠血清丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide Dismutase,SOD)水平的影响,探讨丹参酮ⅡA磺酸钠对百草枯中毒大鼠氧化应激损伤的保护作用。方法:90只Sprague-Dawley(SD)雄性大鼠随机分成对照组、百草枯组、STS组,每组30只。对照组腹腔注射生理盐水2 ml,百草枯组给予生理盐水稀释百草枯50 mg/kg至2 ml灌胃染毒,STS组在百草枯染毒后给予STS 25 mg/kg腹腔注射,连续注射3天。各组分别于制模后24小时、48小时、72小时分别处死大鼠各10只,心脏取血,酶联免疫法检测血清MDA、SOD浓度。结果:百草枯组大鼠各时间点血清MDA浓度较对照组显著升高,其中在48小时达高峰;STS组大鼠各时间点血清MDA浓度较百草枯组比较呈逐渐下降趋势(P<0.05)。百草枯组大鼠各时间点血清SOD浓度较对照组比较呈逐渐下降趋势;STS组大鼠各时间点血清SOD浓度较百草枯比较呈逐渐升高趋势(P<0.05)。结论:STS对百草枯中毒大鼠的氧化应激损伤有一定的保护作用。
Objective: To observe the effect of sodium tanshinone Ⅱ A sulfonate (STS) on the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in paraquat poisoning rats and to explore the effects of tanshinone Protective effects of Ⅱ A sulfonate on oxidative stress induced by paraquat poisoning in rats. Methods: 90 Sprague-Dawley (SD) male rats were randomly divided into control group, paraquat group and STS group, with 30 rats in each group. The control group received intraperitoneal injection of 2 ml of normal saline, the paraquat group of 50 mg / kg of normal saline diluted paraquat to 2 ml, and the STS group received intraperitoneal injection of STS 25 mg / kg after paraquat exposure. day. The rats in each group were sacrificed at 24 hours, 48 hours and 72 hours after modeling respectively, 10 rats in each group were sacrificed, and their blood samples were collected for determination of serum MDA and SOD by enzyme-linked immunosorbent assay. Results: Serum MDA concentration in paraquat group was significantly higher than that in control group at each time point, reaching the peak at 48 hours. Serum MDA concentration decreased gradually in STS group compared with paraquat group at the same time (P <0.05 ). Compared with the control group, the concentration of SOD in the paraquat group was gradually decreased at each time point. The concentration of SOD in the STS group was gradually increased compared with the paraquat at each time point (P <0.05). Conclusion: STS can protect oxidative stress of paraquat poisoning rats.